Terrestrial Extremophiles as a Potential Source of Novel Antibiotics to  Combat Multi-Drug Resistant Pathogens

Adedotun Adefolalu; Olukayode Orole; Oluwatosin Shittu; Evans Egwim; Peter Abioye; Emmanuel Tope Oluwabusola

doi:10.62050/ljsir2025.v3n2.778

المؤلفون

Adedotun Adefolalu
aadotman@gmail.com

Department of Biochemistry, Faculty of Life Sciences, Federal University Lafia, Nasarawa State, Nigeria; Federal University of Technology, Minna, Nigeria
Olukayode Orole
Department of Microbiology, Faculty of Life Sciences, Federal University of Lafia, Nasarawa state, Nigeria
Oluwatosin Shittu
Department of Biochemistry, Federal University of Technology, Minna, Nigeria
Evans Egwim
Department of Biochemistry, Federal University of Technology, Minna, Nigeria
Peter Abioye
Department of Microbiology, Federal University of Technology, Minna, Nigeria
Emmanuel Tope Oluwabusola
Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, UK

الكلمات المفتاحية:

Resistant pathogens , terrestrial , extremophiles , metabolites , antibiotics

الملخص

Human and animal health are increasingly affected by multidrug-resistant pathogens despite efforts to address the challenge. Research focus, shifted to microorganisms from extreme environments as possible sources of antibiotics and metabolites to halt this menace of resistance in pathogens. This paper explores the rich collection of metabolites produced by these microorganisms, their environment, and their applications. Given their intricate nature, these organisms may harbor mechanisms for producing novel metabolites and antibiotics to combat multidrug-resistant pathogens. We highlight recent advances in the isolation, screening, and characterization of antibiotic-producing extremophiles from their harsh terrestrial environments. Studying the diverse chemical scaffolds and mechanisms of action in these microbes can inform the development of effective antimicrobials. Furthermore, we discuss the challenges and prospects of harnessing the vast array of terrestrial microorganisms in extreme environments to combat antibiotic resistance.

Dimensions

REFERENCES

Sharma, S., Chauhan, A., Ranjan, A., Mathkor, D. M., Haque, S., Ramniwas, S., Tuli, H. S., Jindal, T., & Yadav, V. (2024). Emerging challenges in antimicrobial resistance: Implications for pathogenic microorganisms, novel antibiotics, and their impact on sustainability. Frontiers in Microbiology, 15, 1403168. https://doi.org/10.3389/fmicb.2024.1403168

Reygaert, W. C. (2018). An overview of the antimicrobial resistance mechanisms of bacteria. AIMS Microbiology, 4(3), 482. https://doi.org/10.3934/microbiol.2018.3.482

Dadgostar, P. (2019). Antimicrobial resistance: Implications and costs. Infection and Drug Resistance, 12, 3903–3910.

Marston, H. D., Dixon, D. M., Knisely, J. M., Palmore, T. N., & Fauci, A. S. (2016). Antimicrobial resistance. JAMA, 316(11), 1193–1204.

Bokhary, H., Pangesti, K. N. A., Rashid, H., El Ghany, M. A., & Hill-Cawthorne, G. A. (2021). Travel-related antimicrobial resistance: A systematic review. Tropical Medicine and Infectious Disease, 6(1), 11. https://doi.org/10.3390/tropicalmed6010011

Orellana, R., Macaya, C., Bravo, G., Dorochesi, F., Cumsille, A., Valencia, R., et al. (2018). Living at the frontiers of life: Extremophiles in Chile and their potential for bioremediation. Frontiers in Microbiology, 9, 2309.

Rekadwad, B. N., Li, W. J., Gonzalez, J. M., Devasya, R. P., Bhagwath, A. A., Urana, R., & Parwez, K. (2023). Extremophiles: The species that evolve and survive under hostile conditions. 3 Biotech, 13(9), 316. https://doi.org/10.1007/s13205-023-03733-6

Canganella, F., & Wiegel, J. (2014). Anaerobic thermophiles. Life, 4(1), 77–104. https://doi.org/10.3390/life4010077

Artigue, L., Chavagnac, V., Destrigneville, C., François, D., Lesongeur, F., & Godfroy, A. (2025). Fluid chemistry evolution in deep-sea hydrothermal environments: Unraveling mineral-fluid-microorganism interactions through continuous culture experiment. Deep Sea Research Part I, 218, 104456. https://doi.org/10.1016/j.dsr.2025.104456

Canganella, F., and Wiegel, J. Anaerobic thermophiles. Life. 2014;4(1):77–104.

Dopson, M., González-Rosales, C., Holmes, D. S., & Mykytczuk, N. (2023). Eurypsychrophilic acidophiles: From (meta)genomes to low-temperature biotechnologies. Frontiers in Microbiology, 14, 1149903. https://doi.org/10.3389/fmicb.2023.1149903

Salwan, R., & Sharma, V. (2020). Physiological and biotechnological aspects of extremophiles. Academic Press. https://doi.org/10.1016/C2018-0-03860-8

Neufeld, J., Clarke, A., Morris, G. J., Fonseca, F., Murray, B. J., Acton, E. J., & Price, H. C. (2013). A low temperature limit for life on Earth. PLoS ONE, 8(6), e66207.

Siddiqui, K. S., Williams, T. J., Wilkins, D., Yau, S., Allen, M. A., Brown, M. V., & Cavicchioli, R. (2013). Psychrophiles. Annual Review of Earth and Planetary Sciences, 41, 87–115.

Edbeib, M. F., Wahab, R. A., & Huyop, F. (2016). Halophiles: Biology, adaptation, and their role in decontamination of hypersaline environments. World Journal of Microbiology and Biotechnology, 32, 135. https://doi.org/10.1007/s11274-016-2081-9

Merino, N., Aronson, H. S., Bojanova, D. P., Feyhl-Buska, J., Wong, M. L., Zhang, S., & Giovannelli, D. (2019). Living at the extremes: Extremophiles and the limits of life in a planetary context. Frontiers in Microbiology, 10, 780.

Watterson, W. J., Tanyeri, M., Watson, A. R., Cham, C. M., Shan, Y., Chang, E. B., & Tay, S. (2020). Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes. eLife, 9, e56998.

Rodrigues, C. J., Pereira, R. F., Fernandes, P., Cabral, J. M., & de Carvalho, C. C. C. R. (2017). Cultivation-based strategies to find efficient marine biocatalysts. Biotechnology Journal, 12(7), 1700036.

Roslan, M. A. M., Mohamad, M. A. N., & Omar, S. M. (2017). High-quality DNA from peat soil for metagenomic studies: A mini-review of DNA extraction methods. Science, 1(2), 1–6.

Tahon, G., & Willems, A. (2017). Isolation and characterization of aerobic anoxygenic phototrophs from exposed soils from the Sør Rondane Mountains, East Antarctica. Systematic and Applied Microbiology, 40(6), 357–369.

Baranova, A. A., Alferova, V. A., Korshun, V. A., & Tyurin, A. P. (2020). Antibiotics from extremophilic micromycetes. Russian Journal of Bioorganic Chemistry, 46, 903–971.

Stennett, H. L., Tiwari, K., Williams, S. E., Curnow, P., & Race, P. R. (2020). The extremophilic pharmacy: Drug discovery at the limits of life. In Biotechnological applications of extremophilic microorganisms (pp. 43–72). De Gruyter.

Hui, M. L. Y., Tan, L. T. H., Letchumanan, V., He, Y. W., Fang, C. M., Chan, K. G., & Lee, L. H. (2021). The extremophilic actinobacteria: From microbes to medicine. Antibiotics, 10(6), 682.

Bhattacharya, A., & Gupta, A. (2022). Current trends in applicability of thermophiles and thermozymes in bioremediation. In Microbial extremozymes (pp. 161–176). Academic Press.

Coker, J. A. (2016). Extremophiles and biotechnology: Current uses and prospects. F1000Research, 5.

Fenical, W., Jensen, P. R., Palladino, M. A., Lam, K. S., Lloyd, G. K., & Potts, B. C. (2009). Discovery and development of the anticancer agent salinosporamide A. Bioorganic & Medicinal Chemistry, 17(6), 2175–2180. https://doi.org/10.1016/j.bmc.2008.10.075

O’Connor, E. M., & Shand, R. F. (2002). Halocins and sulfolobicins: The emerging story of archaeal protein and peptide antibiotics. Journal of Industrial Microbiology and Biotechnology, 28(1), 23–31. https://doi.org/10.1038/sj/jim/7000204

Pastor, J. M., Salvador, M., Argandoña, M., et al. (2010). Ectoines in cell stress protection: Uses and biotechnological production. Applied Microbiology and Biotechnology, 86(6), 1645–1658. https://doi.org/10.1007/s00253-010-2538-2

Saiki, R. K., Gelfand, D. H., Stoffel, S., et al. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239(4839), 487–491. https://doi.org/10.1126/science.239.4839.487

Cavicchioli, R., Siddiqui, K. S., Andrews, D., & Sowers, K. R. (2002). Low-temperature extremophiles and their applications. Current Opinion in Biotechnology, 13(3), 253–261. https://doi.org/10.1016/S0958-1669(02)00317-8

Oesterhelt, D., & Stoeckenius, W. (1971). Rhodopsin-like protein from the purple membrane of Halobacterium halobium. Nature, 233, 149–152. https://doi.org/10.1038/233149a0

Raymond, J. A., & DeVries, A. L. (1977). Adsorption inhibition as a mechanism of freezing resistance in polar fishes. Proceedings of the National Academy of Sciences, 74(6), 2589–2593. https://doi.org/10.1073/pnas.74.6.2589

Tian, B., Xu, Z., Sun, Z., Lin, J., & Hua, Y. (2007). Carotenoid 3,4-desaturase is involved in oxidative stress resistance in Deinococcus radiodurans. Microbiology, 153(6), 1642–1652. https://doi.org/10.1099/mic.0.2006/004101-0

Vieille, C., & Zeikus, G. J. (2001). Hyperthermophilic enzymes: Sources, uses, and molecular mechanisms. Microbiology and Molecular Biology Reviews, 65(1), 1–43.

Johnson, D. B., & Hallberg, K. B. (2003). The microbiology of acidic mine waters. Research in Microbiology, 154(7), 466–473. https://doi.org/10.1016/S0923-2508(03)00114-1

Santos, H., & da Costa, M. S. (2002). Compatible solutes of organisms that live in hot saline environments. Environmental Microbiology, 4(9), 501–509. https://doi.org/10.1046/j.1462-2920.2002.00358.x

Don, T. M., Chen, C. W., & Chan, T. H. (2006). Preparation and characterization of poly(hydroxyalkanoate) from Haloferax volcanii. Biotechnology Progress, 22(4), 1133–1139. https://doi.org/10.1021/bp060048t

Banat, I. M., Franzetti, A., Gandolfi, I., et al. (2010). Microbial biosurfactants: Production, applications and future potential. Applied Microbiology and Biotechnology, 87(2), 427–444.

Poli, A., Anzelmo, G., & Nicolaus, B. (2010). Bacterial exopolysaccharides from extreme marine habitats: Production, characterization, and applications. Marine Drugs, 8(6), 1779–1802. https://doi.org/10.3390/md8061779

Horikoshi, K. (1999). Alkaliphiles: Some applications of their products for biotechnology. Microbiology and Molecular Biology Reviews, 63(4), 735–750. https://doi.org/10.1128/MMBR.63.4.735-750.1999

Dadachova, E., & Casadevall, A. (2007). Ionizing radiation: How fungi cope, adapt, and exploit with the help of melanin. Current Opinion in Microbiology, 10(6), 525–531. https://doi.org/10.1016/j.mib.2007.09.013

Flinspach, K., Kapitzke, C., Tocchetti, A., Sosio, M., & Apel, A. K. (2014). Heterologous expression of the thiopeptide antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor requires deletion of ribosomal genes from the expression construct. PLoS ONE, 9(3), e90499.

Taylor, S. D., & Palmer, M. (2016). The action mechanism of daptomycin. Bioorganic & Medicinal Chemistry, 24(24), 6253–6268.

Stadler, M., & Dersch, P. (Eds.). (2016). How to overcome the antibiotic crisis: Facts, challenges, technologies and future perspectives (Vol. 398). Springer.

Wright, G. D. (2014). Something old, something new: Revisiting natural products in antibiotic drug discovery. Canadian Journal of Microbiology, 60(3), 147–154.

Richardson, L. A. (2017). Understanding and overcoming antibiotic resistance. PLoS Biology, 15(8), e2003775.

Lamini, J. N., Nfongeh, J. F., & Orole, O. O. (2018). Extended beta-lactamase (ESBL) producing Salmonella typhi from presumptive typhoid patients in Nasarawa State, Nigeria. Greener Journal of Epidemiology and Public Health, 6(3), 80–86.