Characterization and Deployment of Zinc Oxide Nanoparticles of Edible Mushroom with Antifungal Activity against Selected Dermatophytic Fungi

Authors

  • Aleruchi Chuku
    Department of Microbiology, Federal University of Lafia, Nigeria
  • Osuyi Gerard Uyi
    Department of Microbiology, Federal University of Lafia, Nigeria
  • Godwin Attah Obande
    Department of Microbiology, Federal University of Lafia, Nigeria
  • Ayomide Hassan Labulo
    Department of Chemistry, Federal University of Lafia, Nigeria
  • Promise Ujunwa Ikechukwu
    Department of Microbiology, Federal University of Lafia, Nigeria

Keywords:

Antifungal resistance , ZnO nanoparticles , Dermatophytes , edible mushroom

Abstract

Dermatophytic infections continue to contribute to the burden of superficial fungal infections, hence the need for alternative antifungal agents from natural sources. This study comparatively evaluated the efficacy of the crude extract of Cremini kigali mushrooms and its ZnO nanoparticles against selected dermatophytic fungi. Crude extracts were prepared by maceration and ZnO nanoparticles were synthesized using the green synthesis technique, and characterized. Antifungal activity against selected fungi isolates was assessed using agar well diffusion and broth microdilution. The proximate composition of the mushrooms revealed the presence of protein (28.00%), moisture (26.85%), carbohydrates (20.65%), crude fiber (11.50%), ash (9.00%), and crude fat (4.00%). The GC–MS spectral profile of the ethanol extract revealed the presence of diverse bioactive metabolites. Synthesis of ZnO nanoparticles was confirmed through UV spectroscopy with absorption band in the ultraviolet region at 350–380 nm. The FTIR spectrum revealed the presence of functional groups such as O-H, C-H, C=O, and C-N. TEM micrographs revealed the nanoparticles to be spherical in shape with a size ranging from 15 to 45 nm. At a concentration of 500 mg/ml, the antifungal efficacy of the nano-synthesized extract was higher, with the formation of inhibition zones measuring 27.00mm ± 2.00. Results obtained on the minimum inhibitory concentration and minimum fungicidal concentration revealed the antifungal efficacy of the crude extract on test fungi at a concentration of 31.25-6.25 mg/ml and 31.25 mg/ml respectively for the ZnO nanoparticles, indicating the enhanced antifungal efficacy of the nano-synthesized extract compared with the crude extract.

Dimensions

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cover

Published

2026-04-10

How to Cite

Characterization and Deployment of Zinc Oxide Nanoparticles of Edible Mushroom with Antifungal Activity against Selected Dermatophytic Fungi. (2026). Lafia Journal of Scientific and Industrial Research, 4(1), 132-142. https://doi.org/10.62050/ljsir2026.v4n1.786

Issue

Volume 4, Issue 1 (April, 2026), Lafia Journal of Scientific and Industrial Research (LJSIR)

Section

Life Sciences
Copyright & Licensing

Copyright (c) 2026 Aleruchi Chuku , Osuyi Gerard Uyi, Godwin Attah Obande , Ayomide Hassan Labulo, Promise Ujunwa Ikechukwu (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

Characterization and Deployment of Zinc Oxide Nanoparticles of Edible Mushroom with Antifungal Activity against Selected Dermatophytic Fungi. (2026). Lafia Journal of Scientific and Industrial Research, 4(1), 132-142. https://doi.org/10.62050/ljsir2026.v4n1.786

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