Isolation and Characterization of Phosphorus Solubilizing Bacteria from Millet Rhizosphere in Lafia metropolis

Authors

  • Osuyi Gerard Uyi
    Department of Microbiology, Federal University of Lafia
  • Bashiru Eya Sani
    Department of Microbiology, Federal University of Lafia
    https://orcid.org/0000-0002-2983-3861
  • Olukayode Orole
    Department of Microbiology, Federal University of Lafia
  • Gift Chidiebere Ejindu
    Department of Microbiology, Federal University of Lafia
  • Afamefuna Okafor Dunku
    Department of Microbiology, University of Benin
  • Aleruchi Chuku
    Department of Microbiology, Federal University of Lafia
  • Peter Uteh Upla
    Department of Microbiology, Federal University of Lafia

Keywords:

Phosphorus , Phosphorus solubilizing bacteria , plant growth promotion , rhizosphere , millet , soil

Abstract

Phosphorus is an essential, but often unavailable nutrient for plant growth in the soil due to its low solubility. Phosphorus solubilizing bacteria (PSB) convert insoluble forms of phosphorus into soluble forms that can be taken up by plants. The study aimed to isolate and characterize PSB from the rhizosphere of millet plants in Lafia, and to evaluate their potential for improving soil fertility and crop productivity. Soil samples were collected from four different locations and plated on Pikovskaya's agar medium. PSB colonies were identified based on their ability to produce clear zones around them. The PSB isolates were further characterized by morphological, biochemical, and molecular methods. The phosphate solubilizing activity and plant growth-promoting traits of the PSB isolates were also assessed. The PSB isolates were tested for their effect on the growth of millet seedlings in pots. The results showed that 16 PSB isolates belonging to four genera (Bacillus, Pseudomonas, Enterobacter, and Klebsiella) were obtained from the soil samples. The PSB isolates exhibited varying degrees of phosphate solubilizing activity, ranging from 11.2 to 81.6 mg/L. The PSB isolates also showed positive results for the production of indole acetic acid, ammonia, hydrogen-cyanide, and siderophore. The PSB inoculation significantly increased the shoot and root lengths, and fresh and dry weight of the millet seedlings. The study concluded that the PSB isolates from the millet rhizosphere in Lafia could enhance soil phosphorus availability and millet growth. The study recommended further research on the field application and optimization of the PSB inoculation.

Dimensions

Billah, M., Khan, M., Bano, A., Hassan, T. U., Munir, A. and Gurmani, A. R. (2019). Phosphorus and phosphate solubilizing bacteria: Keys for sustainable agriculture. Geomicrobiology Journal, 36, 904–916. https://doi.org/10.1080/01490451.2019.1654043

Pacheco, I., Ferreira, R., Correia, P., Carvalho, L., Dias, T. and Cruz, C. (2021). Microbial consortium increases maize productivity and reduces grain phosphorus concentration under field conditions. Saudi Journal of Biological Sciences, 28, 232. https://doi.org/10.1016/j.sjbs.2020.09.053

Prasad, R. and Chakraborty, D. (2019). Phosphorus basics: Understanding phosphorus forms and their cycling in the soil. Crop Production, Alabama A&M & Auburn Universities Extension, ANR 2535. https://www.aces.edu/blog/topics/crop-production/understanding-hosphorus-forms-and-their-cycling-in-the-soil/

Sharma, S. B., Sayyed, R. Z., Trivedi, M. H. and Gobi, T. A. (2013). Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. Springer Plus, 2, 587. https://doi.org/10.1186/2193-1801-2-587

Penn, C. and Camberato, J. (2019). A critical review on soil chemical processes that control how soil ph affects phosphorus availability to plants. Agriculture, 9, 120. https://doi.org/10.3390/agriculture9060120

Qiao, J., Yu, X., Liang, X., Liu, Y., Borriss, R. and Liu, Y. (2017). Addition of plant-growth-promoting bacillus subtilis PTS-394 on tomato rhizosphere has no durable impact on composition of root microbiome. BMC Microbiology, 17, 131. https://doi.org/10.1186/s12866-017-1039-x

Wu, F., Li, J., Chen, Y., Zhang, L., Zhang, Y., Wang, S., Shi, X., Li, L. and Liang, J. (2019). Effects of phosphate solubilizing bacteria on the growth, photosynthesis, and nutrient uptake of camellia Oleifera abel. Forests, 10, 348. https://doi.org/10.3390/f10040348

Zheng, M. M., Wang, C., Li, W. X., Song, W. F. and Shen, R. F. (2019). Soil nutrients drive function and composition of PhoC-Harboring bacterial community in acidic soils of Southern China. Frontiers in Microbiology, 10. https://doi.org/10.3389/fmicb.2019.02654

Mohamed, A. E., Nessim, M. G., Abou-el-seoud, I. I., Darwish, K. M. and Shamseldin, A. (2019). Isolation and selection of highly effective phosphate solubilizing bacterial strains to promote wheat growth in Egyptian calcareous soils. Bulletin of the National Research Centre, 43, 203. https://doi.org/10.1186/s42269-019-0212-9

Ibrahim, M., Iqbal, M., Tang, Y. T., Khan, S., Guan, D. X. and Li, G. (2022). Phosphorus mobilization in plant–soil environments and inspired strategies for managing phosphorus: A review. Agronomy, 12, 2539. https://doi.org/10.3390/agronomy12102539

Kumar, S., Suyal, D. C., Dhauni, N., Bhoriyal, M. and Goel, R. (2014). Relative plant growth promoting potential of himalayan psychrotolerant pseudomonas Jesenii strain MP1 against native Cicer arietinum (L.)., Vigna Mungo (L.) Hepper; Vigna radiata (L.) Wilczek., Cajanus cajan (L.) Mill sp. and Eleusine coracana (L.) gaertner. Afri. J. of Microbio. Res., 8, 3931–3943. https://doi.org/10.5897/AJMR2014.7035

Aliyat, F. Z., Maldani, M., El Guilli, M., Nassiri, L. and Ibijbijen, J. (2022). Phosphate-solubilizing bacteria isolated from phosphate solid sludge and their ability to solubilize three inorganic phosphate forms: Calcium, iron, and aluminum phosphates. Microorganisms, 10, 980. https://doi.org/10.3390/microorganisms10050980

Abderrazak, R., Nassiri, L. and Ibijbijen, J. (2015). Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of Faba bean (Vicia faba L.) in Meknes Region, Morocco. British Microbiology Research Journal, 6, 247–254. https://doi.org/10.9734/BMRJ/2015/14379

Yazdani, M., Bahmanyar, M. A., Pirdashti, H. and Ali Esmaili, M. (2009). Effect of phosphate solubilization microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of corn (Zea mays L.). Int. J. of Agric. and Biosystems Engr., 3, 50–52. https://publications.waset.org/12844/effect-of-phosphate-solubilization-microorganisms-psm-and-plant-growth-promoting-rhizobacteria-pgpr-on-yield-and-yield-components-of-corn-zea-mays-l

Pikovskaya, R. I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Mikrobiolgya, 17, 362–370. https://www.scienceopen.com/document?vid=44a8aa7f-fad0-4038-a97b-a766d1a3b6fb

Sharon, J. A., Hathwaik, L. T., Glenn, G. M., Imam, S. H. and Lee, C. C. (2016). Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth. Journal of Soil Science and Plant Nutrition, 16, 525–536. https://doi.org/10.4067/S0718-95162016005000043

Viruel, E., Erazzú, L. E., MartínezCalsina, L., Ferrero, M. A., Lucca, M. E. and Siñeriz, F. (2014). Inoculation of maize with phosphate solubilizing bacteria: Effect on plant growth and yield. Journal of Soil Science and Plant Nutrition, 14, 819–831. https://doi.org/10.4067/S0718-95162014005000065

Gupta, R., Kumari, A., Sharma, S., Alzahrani, O. M., Noureldeen, A. and Darwish, H. (2022). Identification, characterization and optimization of phosphate solubilizing rhizobacteria (PSRB) from rice rhizosphere. Saudi Journal of Biological Sciences, 29, 35–42. https://doi.org/10.1016/j.sjbs.2021.09.075

Ummara, U., Noreen, S., Afzal, M. and Ahmad, P. (2021). Bacterial bioaugmentation enhances hydrocarbon degradation, plant colonization and gene expression in diesel-contaminated soil. Physiologiaplantarum, 173, 58–66. https://doi.org/10.1111/ppl.13171

Abhilash, P. C., Dubey, R. K., Tripathi, V., Gupta, V. K. and Singh, H. B. (2016) Plant growth-promoting microorganisms for environmental sustainability. Trends in Biotechnology, 34, 847–850. https://doi.org/10.1016/j.tibtech.2016.05.005

Ma, M., Jiang, X., Wang, Q., Guan, D., Li, L., Ongena, M. and Li, J. (2018). Isolation and identification of PGPR strain and its effect on soybean growth and soil bacterial community composition. International Journal of Agriculture and Biology, 20, 1289–1297. https://doi.org/10.17957/IJAB/15.0627

Rawat, P., Das, S., Shankhdhar, D. and Shankhdhar, S. (2020). Phosphate-solubilizing microorganisms: Mechanism and their role in phosphate solubilization and uptake. Journal of Soil Science and Plant Nutrition. https://doi.org/10.1007/s42729-020-00342-7

Ali, S. and Xie, L. (2020). Plant growth promoting and stress mitigating abilities of soil born micro-organisms. Recent Patents on Food, Nutrition & Agriculture, 11, 96–104. https://doi.org/10.2174/2212798410666190515115548

Amri, M., Rjeibi, M. R., Gatrouni, M., Mateus, D. M. R., Asses, N., Pinho, H. J. O. and Abbes, C. (2023). Isolation, identification, and Characterization of phosphate-solubilizing bacteria from Tunisian soils. Microorganisms, 11, 783. https://doi.org/10.3390/microorganisms11030783

Mohamed, T. A., Wu, J., Zhao, Y., Elgizawy, N., El Kholy, M., Yang, H., Zheng, G., Mu, D. and Wei, Z. (2022). Insights into enzyme activity and phosphorus conversion during kitchen waste composting utilizing phosphorus-solubilizing bacterial inoculation. Bioresource Technology, 362, 127823. https://doi.org/10.1016/j.biortech.2022.127823

Pande, A., Pandey, P., Mehra, S., Singh, M. and Kaushik, S. (2017). Phenotypic and genotypic characterization of phosphate solubilizing bacteria and their efficiency on the growth of maize. J. of Genetic Engr. and Biotech., 15, 379–391. https://doi.org/10.1016/j.jgeb.2017.06.005

Chen, Q. and Liu, S. (2019). Identification and characterization of the phosphate-solubilizing bacterium Pantoea sp. S32 in reclamation soil in Shanxi, China. Frontiers in Microbiology, 10, 440617. https://doi.org/10.3389/fmicb.2019.02171

Front

Published

2024-02-21

How to Cite

Isolation and Characterization of Phosphorus Solubilizing Bacteria from Millet Rhizosphere in Lafia metropolis. (2024). Lafia Journal of Scientific and Industrial Research, 2(1), 23-28. https://doi.org/10.62050/ljsir2024.v2n1.235

Issue

Volume 2, Issue 1 (April, 2024), Lafia Journal of Scientific and Industrial Research (LJSIR)

Section

Life Sciences
Copyright & Licensing

Copyright (c) 2024 Lafia Journal of Scientific and Industrial Research

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite

Isolation and Characterization of Phosphorus Solubilizing Bacteria from Millet Rhizosphere in Lafia metropolis. (2024). Lafia Journal of Scientific and Industrial Research, 2(1), 23-28. https://doi.org/10.62050/ljsir2024.v2n1.235

Most read articles by the same author(s)

1 2 > >> 

Similar Articles

11-20 of 34

You may also start an advanced similarity search for this article.